Can we dilute LysoTracker™ Deep Red (pH sensitive probe dispensed in 1mM solution with DMSO) with citrate buffer ?

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Hello, Why could be the reason for my bands to appear like this?

I am trying to genotype wild type/mutant mice. I used tail snips and 4 different primers

24 April 2022 1,219 2 View

How can I interpret Wilcoxon Rank Sum and Signed Rank Test for Clustered Data?

I have a continuous variable in my dataset and it is not normally distributed. I wanted to check if the continuous variable is different from zero. The observations are not independent. There are...

18 October 2021 4,397 31 View

How to carry out CFD simulation of evaporation and condensation process ?

Give details of CFD simulations procedure step by step. For natural circulation and forced circulation flow problem.

09 July 2021 6,837 5 View

What is the relation of CORONA virus with respective Temperature, immunity, social isolation, Age, gender and blood group?

If we read ongoing research, news, news articles, etc different people are writing different opinions on COVID-19 as follows: 1. It has a relation with temperature ( As it grows very fast below...

03 May 2020 7,726 5 View

Regarding control strategy of inverter in solar grid tied inverter ?

I am working on 5kW grid tied inverter. I have performed detailed simulation of the system in matlab simulink. Simulation was done for the case of increase in active power i.e. increase in...

28 January 2020 790 1 View

How to increase antibody penetration in 3D structures formed in collagen gels?

I have intricate mouse mammary tissue organoids that have grown in 3mg/ml rat tail collagen. I would like to do immunofluorescence staining with some specific antibody. I have tried incubating...

04 August 2019 9,509 3 View

Is Nickel a biocompatible material?

As iron is FDA proved material which can be studied for biocompatible test. In that case can nickel be a biocompatible material?

30 July 2018 1,847 3 View

How do you save data generated from PD Quest software?

I am having an issue with storing data generated using Bio-rad's PD Quest software. Once I am done with 2D electrophoresis analysis, I am unable to export my "Experiment Summary Report"....

15 May 2018 9,233 1 View

Please let me know where do I get a bore/tubewell litholog for Saharanpur, Uttar Pradesh?

Required for information on vertical soil profile and aquifer of the region. I could not find CGWB Ground Water Report for Saharanpur district.

24 March 2018 4,265 4 View

Are there any algorithms similar to random forest algorithm?

Are there any algorithms similar or better than random forest algorithm for prediction and classification

21 February 2016 9,209 6 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View