It is generally known that dyes are the coloring material, organic in nature and used in many industries like textile industry, leather industry etc. and wastewater generated from these industries contain dyes which may create toxicity in the environment as these dyes are sometime persistent in natural environment. Therefore, it is required to detect these dyes in the industrial wastewater so that their degradation by microbes can be explored.
Dr. Saxena,
You can use FTIR. It could be used to identify the structure of the dyes in wastewater.
If you want to investigate the degradation of dyes by microbes, the quantitative determination such as TOC in wastewater may be conducted.
Visible spectroscopy could be used for the detection of dye concentration.
If you just want to follow the concentration, visible sprectoscopy is largely enough.
If you want to determine the dye, FTIR and mass spectroscopy can help you.
Anyway, don't forget that microorganism don't always degrade completely the dye. It can only play a role on the chromophore part of the molecule. The color disappear but not completely the toxicity.
You can measure the Color Index of the waste water following the APHA methods to evaluate the degree of dye degradation. For dye determination, FTIR and mass spectroscopy is required.
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