When i run adfr autodock, give this error, permission denied, how can i resolve it?

hi. i run adfr for docking purpose but give permission denied error and i dont know how can i resolve it please guide me.

04 May 2018 1,228 1 View

Where i can find mdp file for ions current calculation in gromacs?

please guide me

03 April 2018 8,151 0 View

How can i view xpm file?

i play simulation and for review of fluctuation and must view xpm file and use from xview for this file but this program unsupported image type.how i can this type file?

31 December 2017 5,347 0 View

How can i understand my simulation how much time need?

hi i play simulation by gromacs. i run nvt and npt and my system equilibrate. how can i understand my simulation how much time need?

31 December 2017 5,711 3 View

Why is different between temperature plot of nvt and npt?

hi i play MD simulation but, between temperature of nvt and npt is different.is this normal? if no whats factor of this? and why in this pictures rmsd in nvt is different to rmsd in npt state?

31 December 2017 975 5 View

Salam aya kasi midone dakhel tehran aya ostadi hast ke gromacs ra amozesh bede?

salaam dostan man danshjoye olompezeshki tehran hastam mikham bebinam kodom ostad dakhel kodom daneshgah dar tehran gromacs ra dars mide khastam beram sare klasesh?

31 December 2017 7,279 0 View

No title

for secondary structure analysis by gromacs 5.07 i need to install dssp but i dont know how i can install .please guide me

31 December 2017 5,876 4 View

What is the link between replication suppress and protein expression,i need to articles

05 June 2017 1,771 1 View

Hi.does anyone have mae11 antibody sequence?i need it

this antibody trend attach to IgE

05 June 2017 2,393 0 View

The best performance in gromacs

hi dear friends. i run a simulation on my server. my server has 8 CPU core and 640 GPU core, but during of simulation don't used from 100 percent power of CPU core. how can i resolve it?

01 January 1970 2,540 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

No title

23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View