When i run adfr autodock, give this error, permission denied, how can i resolve it?

hi. i run adfr for docking purpose but give permission denied error and i dont know how can i resolve it please guide me.

04 May 2018 1,228 1 View

Where i can find mdp file for ions current calculation in gromacs?

please guide me

03 April 2018 8,151 0 View

How can i view xpm file?

i play simulation and for review of fluctuation and must view xpm file and use from xview for this file but this program unsupported image type.how i can this type file?

31 December 2017 5,347 0 View

How can i understand my simulation how much time need?

hi i play simulation by gromacs. i run nvt and npt and my system equilibrate. how can i understand my simulation how much time need?

31 December 2017 5,711 3 View

Why is different between temperature plot of nvt and npt?

hi i play MD simulation but, between temperature of nvt and npt is different.is this normal? if no whats factor of this? and why in this pictures rmsd in nvt is different to rmsd in npt state?

31 December 2017 975 5 View

Salam aya kasi midone dakhel tehran aya ostadi hast ke gromacs ra amozesh bede?

salaam dostan man danshjoye olompezeshki tehran hastam mikham bebinam kodom ostad dakhel kodom daneshgah dar tehran gromacs ra dars mide khastam beram sare klasesh?

31 December 2017 7,279 0 View

No title

for secondary structure analysis by gromacs 5.07 i need to install dssp but i dont know how i can install .please guide me

31 December 2017 5,876 4 View

Plasmid vector WITH SIGNAL PEPTIDES?

hi i need to some vector which contain signal peptides for example ompA or pELB IN SNAP GENE.

09 October 2017 1,637 0 View

What is the link between replication suppress and protein expression,i need to articles

05 June 2017 1,771 1 View

The best performance in gromacs

hi dear friends. i run a simulation on my server. my server has 8 CPU core and 640 GPU core, but during of simulation don't used from 100 percent power of CPU core. how can i resolve it?

01 January 1970 2,540 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View