We conducted a laboratory experiment (two trials) on the effect of plant extract against rice blast fungus. The study involved 0, 25, 50, 75, and 100% concentrations of ethanolic extracts against conidia of Pyricularia grisea. We found out that 25% was more effected than the rests of the concentrations used. Our question is why? We always thought that higher concentrations are more effected than the lower concentration. But our findings were different. Can someone help us explain why this happened? Your comments are very valuable. Thanks
Dindo
It is quite common in biological system that a smaller concentration may show a higher activity than a higher one. In this case, it is toxicity to fungi.
First, you need to make sure that what you saw is a genuine effect not an experimental artefact. I am sure you did this but check again to question the vehicle you use when applying the extract, etc and establish the treatments are the same in all doses.
Second, a higher activity does not necessarily statistically significant, so do a statistical analysis and establish whether the effect of 25% treatment was indeed significantly different from the rest. If not, it was just a chance.
Third, if you still see a valid difference, then you come to explain by components of your plant extract with different pharmacological effects. This in some case could be additive, synergistic or ANTAGONISTIC.
dear autor
généralement, on assiste à un effet dose dépendant. est ce que vous avez répeter l'experience
Yep. Thanks for all comments. Are there any references you know who have similar results as our experiment? Thanks
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