Does anyone has experience in trying to formulate particles that include an extract as the active substance? I don't know how to quantify the active extract in the drug release test?
Thank you!
Dear Alexandra,
Apply the same principle of qualification of your unknown samples in this case too. I assumed that u already developed and validated qualification method like HPLC or LC-MS for ur formulation. If that is correct, then use it to determine the amount of the API release at different time interval during or after ur release study.
I agree with the suggestion of the colleagues. You should monitor the concentration of the plant marker during your experiment using a methodology with adequate selectivity to separate the analyte from the contaminants.
I suggest you read the reference indicated below:
Article Versatile chromatographic method for catechin determination ...
Hello,
- development of an analytical method for the assay content determination of one or several markers of the extract (chemical markers or related to activity) once included into microparticles
- in vitro drug release test: to perform a dissolution test with your formulated microparticles and to assay the marker(s) over time with the developped method
Good luck!
Dear Alexandra,
First give importance in quantifying the active substance in the extract. You can go for HPLC, LC-MS or basic UV-spec based on your compound nature. Then you can go for in-vitro release studies in simulation conditions.
If u already did the development and validation then go for Assay method by using dissolution tester and calculate the concentration & % of drug released in particular spiking by comparing with standard drug concentration.
First, what's the question you want to answer by studying in vitro release of those compounds? Is it really that important?
Thank you for the answers, I don't know what marker to choose to quantify because my extract has many constituents that are important for the activity.
I guess you should characterize your extract first, for example by HPLC and then see there is a major compound or a mix of compounds with the same characteristic. In vitro release is not a must in microparticle formulation unless you want to understand leakege risk during storage.
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