Should GFP sequence be cut together with the IRES sequence?

Hello. I have a viral plasmid which contains a bicistronic region: CMVprmtr-GFP-IRES-PuroR. I would like to cut out the GFP sequence to get rid of the green fluorescence. In this case, would it be...

02 February 2021 1,644 7 View

Examples of SWAT used in water management in operational practice?

Do you know any examples when the hydrological model made using SWAT became some software product used in operational practice for water management (cases of the water reservoirs management are...

04 June 2020 4,191 1 View

What are these cells with highly condensed basophilic nuclei and a light cytoplasm between proximal tubule epithelial cells in the zebrafish kidneys?

Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...

03 June 2020 4,093 5 View

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home?

Can anyone suggest me any soft for chromatograms viewing and processing (UV MS PDA) which I could use at home (freeware)? Chromatograms were made by Waters UPLC I-Class PDA-MS. At the workplace,...

14 May 2020 5,383 3 View

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It is quite difficult to find these enzyme preparations at that moment. Can anyone tell me who I can contact on this issue with?

29 February 2020 2,130 3 View

How to dilute fish blood plasma?

We are encountered with a phenomenon when T3 FREE was estimated in fish plasma by ELISA (DRG tests): some samples are shown no significant differences in total and diluted plasma (in PBS pH7.4,...

29 September 2019 8,819 3 View

Does anybody know a database on fluorescent properties of chemical compounds?

Does anybody know a database on fluorescent properties of chemical compounds? There are many papers describing flurescent properties of various compounds. Is there any database on fluorescent...

06 August 2019 9,239 3 View

How shoud one switch off a rotary vane pump?

How shoud I switch off a rotary vane pump? Should I initially turn off the pump and then open the shutter to normalize the pressure? Or the order of the actions should be vice versa?

06 August 2019 9,982 3 View

Can anyone verify whether this is an artifact or a point mutation in these Sanger sequencing results?

Hello! I have really strange results in my sequencing chromatograms. The goal was to analyze a point mutation which is heterozygous (DNMT3A gene, in position R882). So I performed a Sanger...

17 May 2019 7,386 3 View

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

Good day! Can someone help me? How can the mixing speed be taken into account in the mathematical model of diffusion in a liquid medium?

19 March 2019 7,162 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View