What is the unit of extinction coefficient?

Hi dear researchers Is it right use 1/mmol unit for extinction coefficient (molar absorption coefficient) instead of 1/mM? (I see this in some atticles)

11 December 2018 3,989 4 View

Why DNA polymerase require a primer but RNA polymerase does not?

Dear researchers Why does DNA polymerase need an RNA primer? but RNA polymerase don't? What happened in catalytic site?

05 June 2018 4,919 0 View

What is the role of polyubiquitin in DNA repair?

Dear researchers I want to know polyubiquitin roles in DNA repair .(briefly)

04 May 2018 8,492 0 View

What is metagenome?

Hi dear researchers I Want to know about metagenome and metataxonomic (definitions and applications). Why we need metagenomics? How metagenomics differ from genomics?

03 April 2018 3,299 0 View

How many torsion angles(Dihedral angle) are there in nucleotide?

Dear researchers, I want to know about torsion angles in nucleotide. Can someone help me?

31 December 2017 2,982 0 View

Does H2O2 have oxidation effects on the nanomaterials?

Dear researchers, Nanomaterials such as nanotubes and nanoporouses are used for immobilization of peroxidase enzymes. H2O2 is one of the Horseradish peroxidase enzyme substrates. Does oxidation...

31 December 2017 2,653 0 View

How Recombinant Insulin is produced?

Dear researchers, I want to know about methods of producing recombinant insulin. Could anyone help me with some literature. Thanks in advance

11 December 2017 2,956 4 View

How sulfide concentration is measured by standard spectrophotometric method?

Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...

10 November 2017 5,111 3 View

How gene expression can be regulated by mRNA structure?

Hi dear researchers According to this sentence "mRNA Spatial shape can effect on gene expression even the amount of gene expression" I waned to know how gene expression can be regulated by mRNA...

09 October 2017 3,209 4 View

How biological element attach to surface in biosensors?

I study a bout biosensors and bioreceptors... I am new biochemistry student...and I want to know a bout surface attachment of the biological element in biosensors... it is mystery for me ...

09 October 2017 1,228 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View