Does small quantity of crude phytochemical(100ul per well) used in microplate reader sufficient to quantitative the gallic acid quantitative of phenole content instead of using single cuvette tools which may time and labour consuming and advantage of standard curve ploting is also gained?
Dear Researcher,
Following links may be useful for you.
http://scidoc.org/articlepdfs/IJFS/IJFS-2326-3350-03-801.pdf
file:///C:/Users/pharmacy/Downloads/molecules-19-09173-v2.pdf
Dear Yahya Alsalhi,
D you want to quantify gallic acid or gallic acid equivalent phenolic content of crude phytochemical?
If you are looking for gallic acid equivalent quantification, the concentration that you have mentioned (100uL) is well enough for a crude extract. However, the concentration of gallic acid equivalence will depend on the stock concentration that you make for the analysis. In some cases, even the lowest concentration of 5 uL can also produce result with a stock concentration of 1:1.
For sure, you can save time and labour.
Hope this will help you
Good Luck!
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