Is the vector backbone of pGEMHE plasmid (expressed in Xenopus oocyte) commercially available?
I want the vector map without any insert. I found out the map of pGEMHE with different inserts in Addgene but the backbone is not mentioned.
I have tried In-fusion cloning few times and it used to work in my hand. Suddenly, after few months when I again wanted to do some more cloning, it is not working as in I am not getting any clones...
18 July 2021 9,849 3 View
I have been trying to image my Western blot using the Fluorescent dye from Biorad called western C protein ladder (Catalog number : #161-0376). There are fluorescent bands of 2 colours : Red...
08 September 2020 4,308 0 View
This is the image of DNA agarose gel where I have loaded the PCR reaction mixture. I see a trail in my DNA gel that starts from the lane. It can't be the non-specific PCR products as the trail is...
01 February 2020 2,836 2 View
I have done a ligation reaction and transformed DH5alpha cells using the ligation mixture. I got colonies in the amp+ plates and so inoculated those in the liquid broth containing ampicillin (of...
01 January 1970 6,089 3 View
I want to express 4 genes in a single cell and so will have to clone all the 4 genes in a vector. During the expression, I want all the proteins to be expressed in the same amount, so I do not...
01 January 1970 6,078 2 View
01 January 1970 8,229 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View
I am currently using a map downloaded from https://gadm.org/maps/DEU/bayern.html and I would like to assign the value of a variable to each cell with R. I was also trying to get this information...
15 July 2024 8,844 1 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View