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I have tried In-fusion cloning few times and it used to work in my hand. Suddenly, after few months when I again wanted to do some more cloning, it is not working as in I am not getting any clones...
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I have been trying to image my Western blot using the Fluorescent dye from Biorad called western C protein ladder (Catalog number : #161-0376). There are fluorescent bands of 2 colours : Red...
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This is the image of DNA agarose gel where I have loaded the PCR reaction mixture. I see a trail in my DNA gel that starts from the lane. It can't be the non-specific PCR products as the trail is...
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I have done a ligation reaction and transformed DH5alpha cells using the ligation mixture. I got colonies in the amp+ plates and so inoculated those in the liquid broth containing ampicillin (of...
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I want to express 4 genes in a single cell and so will have to clone all the 4 genes in a vector. During the expression, I want all the proteins to be expressed in the same amount, so I do not...
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Is the vector backbone of pGEMHE plasmid (expressed in Xenopus oocyte) commercially available? I want the vector map without any insert. I found out the map of pGEMHE with different inserts in...
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