I'm currently extracting RNA from skin biopsies. Typically, I first digest the skin in Trizol using 5mm beads and a TissueLyser. The samples are left on the TissueLyser until they appear completely digested.

Today, I had an unexpected problem. the tissues appeared fully digested, but after centrifuging for phase separation, I found pellets in the bottoms of the tubes. Presumably this is undigested tissue. As a test, I carried on with the extraction protocol and unsurprisingly found low RNA recovery.

As the tissue is in limited supply, is there any way to redigest those pellets? Maybe by resuspending them in fresh Trizol? Are the ok the be left in th bottom phase of the phase separation while I decide what to do? I feel like this might be hopeless, but worth a try.