Perforin and Granzyme flow cytometry staining in purified T cells?

Alice Sint Thida Bo @Alice-Sint-Thida-Bo

12 November 2019 0 4K Report

I want to activate purified T cells from mouse LN or spleen with CD3/28 or PMA/ionophore and analyze perforin and granzyme expression by intracellular staining with FACS. What's the recommended concentrations of stimulants and the duration of the stimulation? Thanks for your help!

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