Peptide lost during reverse phase HPLC?

More Raheleh Tooyserkani's questions See All

Which analysis in gromacs can determine the validity of a defined topology and show the best topology among different topologies?

Hi , I am a beginner in gromacs. I have made some topology files for graphene sheet with different bonded and non-bonded parameters I found in the literature. Now, how can I choose the best...

08 May 2020 7,509 3 View

How to calculate Kd with flowcytometric analysis?

Hello; I want to calculate Kd value for a FITC-conjugated peptide which specifically binds to a cell receptor. I've studied some protocols, but still confused! Does anyone have any experience or...

02 December 2018 3,385 2 View

What is the optimal concentration of G418 for selection of HEK293-T cells?

I've transfected HEK293-T cells with pCI as a mammalian expression vector containing neomycin gene as a selectable marker. I need to know the most efficient concentration of G418 for cell...

25 April 2015 1,154 4 View

How can I remove the free HC-LC from my antibody?

I have reduced the IgG4 to half antibody using TCEP. I see some (20%) free heavy and light chain (SDS-page), but they are still bound to each other (noncovalently). I see only one peak during...

09 February 2015 1,686 3 View

Sudden stop in recombinant protein expression in BL21?

I have cloned a 25KD fragment in pet32a and I got recombinant expression in BL21. The yield of expression was very good, but suddenly it stopped expressing!I tried transforming new BL21 bacteria...

08 February 2015 3,947 1 View

Is there any simple method to evaluate immune responses in immunized animals?

I have immunized rabbits and mice with DNA and I got no antibody answer in ELISA test! I need to know is there any other method to consider the presence of immune response?! I mean I want to know...

07 February 2015 2,730 5 View

Is Tolerance possible in DNA vaccination?

I'm working on polyclonal antibody production through genetic immunization in rabbits and after 5 month of immunization I got nothing! I need to know is there a problem with Tolerance induction...

01 January 2015 1,772 4 View

What is the exact sequence or structure difference between dimeric and tetrameric form of pyruvate kinase M2 in tumor cells?

Articles have mentioned they used antibodies to specifically detect the dimeric form but there's no sequence difference between dimer/tetramer as far as I know!

13 August 2013 1,940 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View