Peptide lost during reverse phase HPLC?

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Jasim Al-saadi

Hi Raheleh

Can you tell me more information's about the mobile phase you used?

Raheleh Tooyserkani

Hi, the mobile phase is a combination of water and acetonitrile. Acetonitrile starts at he 5% concentration, reaching to 25% after about 30 minutes, then increases to reach 100% during next 30 minutes.

Raheleh Tooyserkani what is the kind of detector you use ?