Can you help me with pharmacopoeia procedure 3.2.1 on arsenic determination?

Hi to all I am writing you in order to get more information about the procedure 3.2.1 on Arsenic determination in glass containers for pharmaceutical use. In the monograph, at the paragraph...

05 June 2018 1,229 3 View

How do you overcome the following issues with S and Os ICP-OES?

hi to all, i'm experiencing several issues with detection of elements such as sulfur and osmium at icp-oes. in particular, i work with an Agilent ICP, and at each analysis, i have to analyse these...

08 September 2017 5,321 3 View

Is it possible to infere from isoelectric point the superficial pH?

hi to all, i would like to estimate the superficial pH on spherical particles having dimension of nanometers. since the surface is regular and omogeneusly coated, i was wandering if could i infere...

02 March 2016 8,923 2 View

No title

02 March 2016 8,187 4 View

How can I stop protein induction?

hi to all, i was wandering if it is enough to pellet cells (e.coli) from the protein induction medium containing iptg and resuspend them in a new medium without inductor to stop the protein...

01 February 2016 3,521 3 View

Is it possible to co-trasform E.Coli bl21 de3 gold with two iptg inducible plasmids?

hi to all, i was wandering if it is possible to transform bl21 de3 cells with two plasmids. the aim is to espress 2 proteins at once. the two plasmids are both iptg inducible, and ampicillin...

01 February 2016 2,988 8 View

Is it strictly necessary the chloramphenicol in LB agar plates for pLysS cells, or the ampicillin is enough?

If I add chloramphenicol only in the overnight liquid culture, is ok? should the chloramphenicol be present also during the induction of the protein? thank you

31 December 2015 1,109 3 View

Could the OD_600 of a bacterial culture be a clue of protein expression?

hi to all, i have noticed that if i incubate two parallel bacterial cultures in the same conditions ( one where i add IPTG for protein induction and another in which I don't add anything) the OD...

31 December 2015 935 7 View

Can glucose polymers be used by E.Coli as carbon Source?

Hi to all, I'm wondering if glucose polymers such cyclodextrins, glycogen and more complex polymers, are metabolized by E.Coli. Thank you

09 October 2015 2,677 4 View

How can I dock a 4Fe-4S cluster into a protein?

hi to all, I'm trying to dock a 4Fe-4S cluster into a protein using the Haddock web-tool. I suppose that the protein I'm using coordinates the cluster by means of specific residues, that I have...

05 June 2015 5,853 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View