7 Questions 26 Answers 0 Followers
Questions related from Farah Deeba
I am doing EMSA by using DIG labelled kit from Roche. I annealed and labelled the oligos according to kit manual, checked labelling efficiency, it was good. Than I did EMSA with nuclear extract...
09 September 2014 7,971 8 View
I did cloning of vector containing a promoter sequence, confirmed it by PCR and plasmid size. Then transfered the gene to expression vector and sent it for sequencing. Whet I got sequencing...
06 June 2013 9,006 7 View
I have extracted nuclear proteins but when I run it on native PAGE and stained with SYBR green, 2 bands of DNA appear while those bands were absent when stained with protein dye. I think there is...
03 March 2013 5,985 4 View
Can we use single stranded probe for EMSA for transcription factor identification?
11 November 2012 1,565 4 View
Have anyone tried EMSA on Agarose? I have started to do EMSA on Agarose and I am confused regarding few things. Is there any specific agarose for Proteins? What protein concentration should be...
10 October 2012 9,780 12 View
I have to identify an ancestral gene from a gene family, which has been duplicated many times and gave rise to a gene family. Please tell me any software which can help me.
09 September 2012 6,600 8 View
I am doing EMSA for the identification of transcription factors. I am using total nuclear proteins. When I run the sample of Protein alone and protein plus DNA sample there is no difference in...
09 September 2012 6,426 12 View
