Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

Which image processing software is the best for gel densitometry?

I am using the "ImageJ" software in order to quantify the protein bands on the SDS-PAGE, but I'm not sure it's the best software for this purpose. Does anyone have the experience of using other...

10 January 2019 2,882 12 View

What is the best method to dilute DNA samples?

I want to create a serial dilution of genomic DNA and plasmid samples for absolute qPCR. In order to get repeatable results in the Real time PCR, I want to do a very accurate dilution. I know that...

25 October 2018 5,282 12 View

A question for writting proposal?

hello, I would like to write a project for DFG. I would like to know that how much DFG support for projects and the duration of projects is limit or not? Also, Can I have your proposal draft?...

01 January 1970 1,609 1 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Three-way robust ANOVA in R?

I am trying to conduct a three-way robust ANOVA in R using the WRS2 package as: t3way(Stem ~ Species*Temperature*Salinity, data = tutto) and there is an error saying : Error in if...

22 February 2021 1,674 8 View

How do I dissolve cannabidiol (CBD) and crude hemp extracts in 1:1:18 ethanol:tween80:0.9% saline for IP injection in mice?

I have tried dissolving CBD and crude hemp extracts in 1:1:18 ethanol: tween80: 0.9% saline. I dissolved the drugs in ethanol first then tween, after 10 mins sonication the solutions looks ok but...

27 January 2021 6,259 3 View

Is there any low-cost filtering system (or other tech.)to lower the salt conc. (salinity) of surface/groundwater for large scale agricultural system??

The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...

18 January 2021 5,015 3 View

Which vegetable crops can tolerate salinity , Except Solanecious crops ?

Except ( Tomato-Brinjal-Capscum-Chilli) .

27 December 2020 8,362 12 View

Soil analysis under salinity and foliar nanoparticles?

I am looking for soil tests including (PH EC and others tests) under salinity and foliar nano .

27 December 2020 6,161 2 View

What is the reason for salinization and land degradation, could it be possible to overcome salinization and land degradation by biological method?

When moisture evaporates from the soil surface, the water evaporates, the salt remains in the surface, which will lead to salinization

20 December 2020 6,143 4 View

No title

16 December 2020 9,657 5 View

How many times should I inject the drug before I take samples for RNA-Sequencing?

I'm going to perform RNA-sequencing of the zebrafish brain with three different treatments, which are naive fish, alcohol withdrawal fish injected with Saline, alcohol withdrawal fish injected...

08 December 2020 4,643 2 View

How to calculate the analytical solution for the HYDRUS-3D model simulation of water and salt movement in soil?

Suppose an agricultural field with uniform soil texture and salinization. The groundwater depth is 20 meters, assuming that the depth of the groundwater and the concentration of salts remain...

25 November 2020 2,382 4 View