Hello everyone
I have a typical question. I am trying to add PEG 5K as a surfactant alongside PVP K25 to make PLGA NPs. I can't understand why when I am trying to wash the particles (centrifugation at 9000rpm for 20min) irreversible aggregates occur. the same experiment was perfectly successful using a combination of PVA and PVP K25
thanks for your help!