We have decided to do PCR-free Whole Genome Sequencing on Illumina sequencers. Will standard Y-shaped adaptors work? or do we need special linear adaptors?
Any advice is appreciated.
Hi researchgate community, I am planing to differentiate neurons from induced pluripotent stem cells and the protocol recommends to add AraC (Cytosine β-D-arabinofuranoside) at some point to kill...
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I have already tried different conditions (temperature, salt concentration, presence of reducing agents, different concentration of protease) but the removal of the tag is always very partial (...
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I performed a PCR on some samples that looked good and only produced 1 band on a gel. After PCR purification with a Qiagen kit following normal procedures, I sent the samples out for sequencing. I...
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I'm sorry if we are supposed to ask only about specific research topics, but lately, I've been wanting to learn a new language that could also help me on my research field. I'm not sure which one...
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I am trying to do an ATACseq library prep on human lymphocytes and keep losing the majority of cell nuclei in aspiration steps. It was suggested I could try using Concanavalin A beads to help...
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I would like to label a short peptide (30 aa) with a fluorescent dye. I'd like to avoid amine modification, since I have 3 lysins and I am afraid that labeling all of them would change the...
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How do I find the right PhD? I'm going crazy about finding a PhD where I can combine the study of marine viruses and their effects on ocean environments (biological pump and biogeochemistry). If...
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I seem to recall it being necessary to completely dry glassware before autoclaving on dry-cycle. However, a peer of mine doesn't think this is necessary and puts still wet glassware to autoclave...
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
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I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
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I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
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I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
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When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
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Gene sequencing related trouble shooting
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In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View