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Questions related from Sélène Tyndale
We have decided to do PCR-free Whole Genome Sequencing on Illumina sequencers. Will standard Y-shaped adaptors work? or do we need special linear adaptors? Any advice is appreciated.
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I am trying to do an ATACseq library prep on human lymphocytes and keep losing the majority of cell nuclei in aspiration steps. It was suggested I could try using Concanavalin A beads to help...
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I am working with several immortalized cell lines and need a protocol to uniquely ID each, perhaps by genotype? SNP array? Anyone have a good SOP for this? or know of a company that provides...
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We are currently using PCR clean-up columns to purify 14Kb circular plasmid DNA & getting low yields in large volumes. We routinely use Magnetic Beads (AmpureXP or Kapa) to clean &...
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