Sélène Tyndale

2 Questions 9 Answers 0 Followers

Questions related from Sélène Tyndale

PCR-free WGS on Illumina, will standard Y-shaped adaptors work? or are linear adaptors needed?

We have decided to do PCR-free Whole Genome Sequencing on Illumina sequencers. Will standard Y-shaped adaptors work? or do we need special linear adaptors? Any advice is appreciated.

25 October 2018 3,466 0 View

Will Concanavalin A beads help precipitate nuclei from lymphocytes?

I am trying to do an ATACseq library prep on human lymphocytes and keep losing the majority of cell nuclei in aspiration steps. It was suggested I could try using Concanavalin A beads to help...

22 March 2018 3,076 5 View