Hey all,
I'm trying to linearize by scaI my plasmid in order to transfect my embryonic stem cell, how can I know for sure that I linearize well on gel ?
yes but if I don't cut 100% of my plasmide and there is circular DNA,supertours DNA and also linearize DNA
how can I know what will be my linearize DNA?
Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...
16 December 2020 7,622 2 View
On Line student doctorant looking for first Master PhD student doctorant as UNICAF now in Materials engineering for international students to study abroad on Line and To Bricks LTGS and To have...
10 December 2020 3,686 3 View
Hello, I have three same papers which have different numbers of citations and different number of readings and I would like to merge these three papers in one paper with taking account of a new...
20 September 2020 5,275 6 View
Hello everyone, I recently started several polysome profiling experiment and something bugs me out. Every protocols I found use 12mL tube. They load their lysate containing ribosomes attached to...
19 September 2020 8,717 10 View
The sensitivity of the technique is important to know how to identify the appearance of cracks
17 August 2020 4,156 9 View
31 May 2020 6,077 6 View
I am looking for an efficient way to collect a ground-nesting bee at its nest, so that when encountering a ground-nesting bee nest, I could determine what species created that nest. Ideally, I was...
21 February 2020 5,269 3 View
While investigating about French women botanists in the XIXth Century I chanced upon two herbarias, made from plants collected in Brazil. I am looking for someone who could help to evaluate the...
07 October 2019 2,560 6 View
Dear all, What are the most appropriate journals to submit a method we developed to estimate the seismic vulnerability index for touristic accommodations ? and its application to a pilot zone of...
08 September 2019 8,878 3 View
At the COP 21, in France, a few years back, an initiative was proposed to increase the carbon content of soils by 0.4% or 4 per 1000 per year. The claim was made that, in so doing, soils could...
28 May 2019 3,080 12 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...
20 February 2021 6,897 1 View
I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...
15 February 2021 7,444 2 View
I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...
14 February 2021 7,800 7 View
I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...
09 February 2021 8,594 3 View
In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...
08 February 2021 2,792 1 View
Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...
07 February 2021 9,530 3 View
I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...
04 February 2021 1,509 3 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View