Hey all,
I'm trying to linearize by scaI my plasmid in order to transfect my embryonic stem cell, how can I know for sure that I linearize well on gel ?
yes but if I don't cut 100% of my plasmide and there is circular DNA,supertours DNA and also linearize DNA
how can I know what will be my linearize DNA?
Some monthes ago on RG I found a nice paper by a bryologist explaining that in a single small tuff of epiphytic mosses different species might co-occur. I remember a plate illustrating type...
01 July 2024 5,102 0 View
Hi everyone, I want to study the translation efficiency of multiple version of the same protein coded by different genotypes. To do so I need to conserve the translation initiation context ->...
29 April 2024 6,779 1 View
plastic revolution?
10 April 2024 4,191 2 View
Hi everyone, everything is in the title question ! To the best of your knowledge, what is the shortest sequence you know that translate into a fluorescent protein that could be used for flow...
07 March 2024 1,762 1 View
Hi everyone, I'm currently working on the HaCaT keratinocyte cell line. I have them in culture under low and high calcium concentration to keep them undifferenciated or differenciated,...
04 March 2024 2,153 0 View
Hello everyone, I use several pcDNA3.1 expression vectors to transfect cells. The vectors were prepared by midi-prep a year ago and diluted in TE buffer. Now that I run new experiments, I...
04 March 2024 1,867 3 View
I work on a MODFLOW Model using GMS to simulate subsurface water exchanges between a small straightened stream and its heavily modified alluvial plain. I’m looking for efficient methods to modify...
09 January 2024 708 0 View
Hello everyone, I'm curently working on HaCaT cells which require low calcium medium to return to undifferentiate state. To do so, I need to chelate my FBS for 1h with chelex resin among other...
12 December 2023 4,975 2 View
In an action-research, our research team is conducting interviews to identify key moments about professional practices in the field of education with participants.
04 December 2023 2,114 1 View
Hello, I was not able to find a natural inhibitor for Fibroblast Activation Protein (FAP) in the banks or in the literature. Even among broad spectrum inhibitors Is there any identified? Thank...
26 November 2023 636 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches
04 August 2024 2,703 6 View
Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...
31 July 2024 8,141 13 View
I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...
30 July 2024 4,267 2 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
Employing a pragmatic inquiry research design, looking for published research using this method, employing qualitative research data collection methods of semi-structured interview and focus...
28 July 2024 540 2 View
First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...
27 July 2024 3,457 3 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Greetings! I am looking for materials on quantitative and qualitative research. What are the methods of collecting information and the principles of their implementation? I would like to make an...
20 July 2024 2,033 7 View