So i need to do a quantification of Theileria annulata and Anaplasma marginale from tick DNA samples. But i am a bit confused when it comes to deciding on using a house keeping gene as most studies I have seen on these said pathogens did not use any.
Mariam Bakshi
28S rRNA and beta actin genes are usually most stable genes. For accuracy, you could try different reference gene primers and select the most stable one for analysis. Granted that the DNA quality is good.
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Firas Alali
28S rRNA and beta-actin genes are usually the most stable genes and common in experiments.
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