Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

How to obtain CMIP6 Global Climate Model dataset?

Hi, I am using hydrological modelling under future climate projection in conparison to cmip5 and cmip6. What are the best sourcesfor GCMs dataset for SSPs scenarios?

26 August 2020 3,703 7 View

Which source is best for vegetation map in south-east Australia and MDB?

I would like to get vegetation type details in the MDB and the Murrumbidgee River catchment area to refer it for satellite image analysis. Please also refer any relevant articles. Thank you all in...

30 March 2020 9,549 3 View

How can I extract temporal and spatial data from satellite image?

I would like to extract temporal and spatial data for LAI from a satellite image.

21 March 2020 1,470 5 View

If failure 1 occurred 5 times in a month consuming 10 hours versus failure 2 which occurred 10 times consuming 5 hours. Which should be prioritized?

Please help me to understand which failure on a production line should be prioritized when comparing failure 1 which occurred 5 times in the month and consumed 10 hours (total) versus failure 2...

03 December 2019 4,782 8 View

What will be the trend of sound speed in higher dimensional compact star modelings?

In many articles I have seen that the sound speed within higher dimensional compact stars are increasing in trend. However, in general relativity, we tried to model compact stars not only obeyed...

19 April 2016 2,244 3 View

Can the sub fields within a field ever be truly autonomous?

If we take the field of formal education and drill down to the sub fields of mainstream and alternative education and we move a student from mainstream into alternative education, homology of...

12 March 2016 1,333 3 View

Does it matter how long my bacteria take to reach the correct OD for RNA extraction?

I am hoping to perform some RT-PCR. However my bacteria has taken longer to reach the correct OD at which I should harvest. Does this reflect in anyway the RNA which I then prep and take forward...

05 February 2013 7,401 4 View

No title

01 March 2021 3,905 5 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

What is this fern?

Please help me to identify this fern species. Thank you in advance.

24 February 2021 2,435 5 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Can medicinal plants really play an inherent rule in the COVID-19 tackle?

Numerous scientific reports cited plant extracts with potential antiviral activity and even anti-COVID-19? Why these findings are neglected? I remember very well the warning released by the WHO to...

17 February 2021 2,141 9 View

Is there any solar electric power plant having the concentrated photovoltaic modules?

The solar photovoltaic (PV) power plant uses commercial (non-concentrated) PV modules which are quite simple in design and reliable. They can work on fixed orientation and don't need the moving...

16 February 2021 3,796 9 View