How to proceed with sds page when protein concentration is negative?

Hi. I measure my protein concentration using Nanodrop. and I get negative value. So, how can I proceed with SDS PAGE then? Thank you.

08 September 2015 2,088 8 View

How do I dissolves aqueous extracts completely?

I used sterile distilled water to dissolves my extracts to get a concentration of 320 mg/ml. However, my aqeuous extracts do not dissolves completely in the distilled water. And when I want to...

08 September 2015 9,796 5 View

Why my resolving buffer form a crystalized like substances when stored at 4 degree?

Every time I store my resolving buffer at 4 degree, the next day a crystallized like substances will appear at the bottom of the bottle. Why is it happening? is it because of pH? I already...

08 September 2015 3,355 5 View

How can my disc diffusion test give negative result but in MIC test give positive result?

i used disc diffusion for screening of antimicrobial activity of a plant extract, and I used concentration range 100 mg/ml to 20 mg/ml. For each concentration, no zone of inhibition were observed...

07 August 2015 717 4 View

How can I calculate the phagocytic assay and the MOI?

I want to use, 1:10 MOI, (cell: bacteria). Assuming that one well of cell is 1 x 10^5, thus, the no of bacteria per well should be 10 x 10^5. The bacteria is V. cholerae, and from my reading, at...

05 June 2015 698 1 View

How to calculate final OD for infection to cell line?

hi. i want to know, how to calculate the final OD of the bacteria, when: OD 600nm~ 0.5 nm, MOI 1: 100 number of cells : 2 X 10^5 per well. What is the correct formula to get the final OD of...

04 May 2015 8,877 1 View

What is the time requirement for drying plant extract in a 50 degree incubator that will affect the extraction result?

02 March 2015 6,863 9 View

What if I skip the filtration step and concentrate the sample during aqueous extraction of fruit pulp?

during boiling process, the extract already become too saturated and difficult to be filtrated. hence, i skip the filtrating and concentrating steps and proceed with the freeze drying step. is...

02 March 2015 4,090 6 View

After drying the sample, how long can it be stored before it will be extracted ?

02 March 2015 2,659 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View