Fairuz Iman

10 Questions 8 Answers 0 Followers

Questions related from Fairuz Iman

How do I dissolves aqueous extracts completely?

I used sterile distilled water to dissolves my extracts to get a concentration of 320 mg/ml. However, my aqeuous extracts do not dissolves completely in the distilled water. And when I want to...

09 September 2015 9,858 5 View

Why my resolving buffer form a crystalized like substances when stored at 4 degree?

Every time I store my resolving buffer at 4 degree, the next day a crystallized like substances will appear at the bottom of the bottle. Why is it happening? is it because of pH? I already...

09 September 2015 3,420 5 View

How to proceed with sds page when protein concentration is negative?

Hi. I measure my protein concentration using Nanodrop. and I get negative value. So, how can I proceed with SDS PAGE then? Thank you.

09 September 2015 2,147 8 View

How can my disc diffusion test give negative result but in MIC test give positive result?

i used disc diffusion for screening of antimicrobial activity of a plant extract, and I used concentration range 100 mg/ml to 20 mg/ml. For each concentration, no zone of inhibition were observed...

08 August 2015 778 4 View

How can I calculate the phagocytic assay and the MOI?

I want to use, 1:10 MOI, (cell: bacteria). Assuming that one well of cell is 1 x 10^5, thus, the no of bacteria per well should be 10 x 10^5. The bacteria is V. cholerae, and from my reading, at...

06 June 2015 774 1 View

How to calculate final OD for infection to cell line?

hi. i want to know, how to calculate the final OD of the bacteria, when: OD 600nm~ 0.5 nm, MOI 1: 100 number of cells : 2 X 10^5 per well. What is the correct formula to get the final OD of...

05 May 2015 8,938 1 View

Parametric test vs non parametric test?

Hi. I would like to ask, why in lab based research, we used parametric test although the data is not normally distributed? Thanks in advanced!

04 April 2015 1,287 0 View

After drying the sample, how long can it be stored before it will be extracted ?

I stored them at -20 degree Celsius. What happens if I store them for a long time before the extraction starts?

03 March 2015 2,711 5 View

What is the time requirement for drying plant extract in a 50 degree incubator that will affect the extraction result?

For example, one day and third day incubation. How can I determine that the extract can be proceed with subsequent procedures? thanks in advanced!

03 March 2015 6,923 9 View

What if I skip the filtration step and concentrate the sample during aqueous extraction of fruit pulp?

during boiling process, the extract already become too saturated and difficult to be filtrated. hence, i skip the filtrating and concentrating steps and proceed with the freeze drying step. is...

03 March 2015 4,147 6 View