It depends what material and technique you use: histological techniques or MRI...?

Dear Karim, First i want to check it theoretically. Like by some parameters as molecular weight, size, groups attached, etc.

There is an interesting book that explains materials and methods for studying the BBB, from Prof Nag Sukriti: "The Blood-Brain Barrier : Biology and Research Protocols".

http://www.springerprotocols.com/BookToc/doi/10.1385/1592594190

patent in Neuroprotection Inc (Greater Boston Area )