How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

How to dissociate and culture retinal cells from mouse eyes?

We have a transgenic mouse line in the laboratory from which we are looking to culture retinal cells from. Our experiments indicate we might be able to produce stable cultures from these cells...

03 January 2021 7,200 6 View

How can i calculate sample size for split mouth design?

i have the formula but i need more help

10 December 2020 6,593 4 View

What is the cause of abnormal breathing in anaesthetized rats?

Is anyone able to tell me why my anaesthetized rats often do a 'double breath', or diaphragmatic breath, like a hiccup (see video). This often begins after 1-2 hours under anesthesia, and can...

06 December 2020 2,514 5 View

Which anesthesia system should I buy to perform stereotaxic surgeries in mice?

I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many...

06 November 2020 3,613 3 View

Problem about rat anesthesia?

I have a problem about rat anesthesia, the situation is that I have Zoletil 50 1mg/ml + xylazine hydrochloride 23.32mg/ml solution that I prepare at the same time from the same source (from the...

07 October 2020 9,140 7 View

How to transfer inter-occlusal space from patients' mouth to the prosthesis laboratory?

Hi, I have a big problem with transferring the inter-occlusal space from the patients' mouth to the laboratory! Every time I receive a fabricated prosthetic dental crown or bridge from the...

31 July 2020 795 2 View

Is food production without soil the solution to feed the increasing mouths?

Land has already become limited and soil fertility, in some places, like the developing and poor nations, is gone. The biggest challenge now is that increasing mouths need to be feed from very...

20 July 2020 4,229 35 View

Why there is a 11 kDa band showing in SDS-PAGE of my IgG1 digestion with Papain?

Hi guys, I am using the papain digestion system to abtain a Fab. Here is my recent result, I got thin bands around 55, 45; thick bands around 30, 25, which may indicate incomplete digestion, but...

27 June 2020 3,882 9 View