Dear colleagues, can you please recommend a pan caspase activity assay/kit to be used for cell lysates (rather than in whole cells in situ)?
Many thanks,
Joanna
Hi Joanna
Selectivity assays for caspases can be initiated with purified enzyme
Check this paper:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=2ahUKEwig8uq45KjlAhXDEVAKHQT4CpEQFjABegQIAhAB&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC2780545%2F&usg=AOvVaw0UYEsR3NMR8rbtbZ-FmKb4"
a cell-permeant pan-caspase inhibitor
Check:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwig8uq45KjlAhXDEVAKHQT4CpEQFjACegQIAxAB&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC3754665%2F&usg=AOvVaw0uxHk0nV35WkyCVnhG08cV"
Assay Caspase activity in 2 hr in cell samples with pan Caspase (active) Red Staining Kit ab65616. For microplate reader or fluor. microscope or flow
Check paper:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=2ahUKEwig8uq45KjlAhXDEVAKHQT4CpEQFjADegQIChAB&url=https%3A%2F%2Fwww.abcam.com%2Fpan-caspase-active-red-staining-kit-ab65616.html&usg=AOvVaw1Btxn-cFXo-YLXwPszl77v"
Pan-caspase irreversible inhibitors that blocked proteolysis of. Htt at caspase-3
Check paper:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&uact=8&ved=2ahUKEwig8uq45KjlAhXDEVAKHQT4CpEQFjAGegQIBBAB&url=http%3A%2F%2Fwww.cell.com%2Fccbio%2Fpdf%2FS1074-5521(10)00399-6.pdf&usg=AOvVaw384nmxg3PWG-5REdHfZuFs"
Thanks. I am not sure if Ab65616 can be used on the cell lysates though?
Cell lysates from the same macrophages
Check paper:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&cad=rja&uact=8&ved=2ahUKEwig8uq45KjlAhXDEVAKHQT4CpEQFjAJegQICBAB&url=https%3A%2F%2Fwww.ahajournals.org%2Fdoi%2Fpdf%2F10.1161%2F01.ATV.0000078602.54433.07&usg=AOvVaw2WJNkLsEaXgdtNG3DEjmzI"
Caspase-3 Activity Assay Kit - Cell Signaling Technology
Check:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=11&cad=rja&uact=8&ved=2ahUKEwig8uq45KjlAhXDEVAKHQT4CpEQFjAKegQIARAB&url=https%3A%2F%2Fwww.cellsignal.com%2Fproducts%2Fcellular-assay-kits%2Fcaspase-3-activity-assay-kit%2F5723&usg=AOvVaw1NvkBTYsmwKD5TWv8acvQm"
= Caspase-9 Activity Fluorometric Assay Kit
= Each of the supplied fluorogenic substrate and the pan caspase inhibitor
Check:
"https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=2ahUKEwjsyJDrlKnlAhUxsaQKHVVrCDE4ChAWMAN6BAgCEAE&url=http%3A%2F%2Fwww.ubpbio.com%2Findex.php%2Fcaspase-9-activity-fluorometric-assay-kit.html&usg=AOvVaw3hgZER8WqQXL186-9gDoYS"
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