I've recorded from neurons in the DMH of the hypothalamus. My evoked currents from my control and stress groups (unpaired t test) also did not pass the normality test, but were corrected with a 1/y transformation.
My paired pulse ratios are also not normalized. I don't expect either my evoked amplitude or paired pulse ratios to be normal but for statistical processes, I understand that a transformation is best.
Is this the right way to go? To transform 1/y for both my evoked and PPR? Because now in a graph, my PPR data is doing to be different than from other studies who present it as simply PPR, not 1/PPR transformation.
Additional information:
My protocol is:
5 min baseline (with 2 evoked currents 50 ms apart)
High frequency stimulation (HFS) Twice at 100Hz for 4 seconds, 20sec apart.
25 min post HFS (with 2 evoked currents 50 ms apart)
Why don`t you try non-parametric Mann-Whitney test? In addition, it appears to me you are comparing more than two.groups, and in that case you cannot use t test nor MW. For that matter, non normal.data can be analyzed by kruskal-wallis and a post-hoc (i use nemenyi).
As already mention use a non-parametrric statistics. No T test can be performed if data are not normal.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Molecular Methods
- Cloning