I am Working on Barcoding of Olive crop. In routine matter the apparatus works fine but i am unable to understand the trouble shoot last day.
Usually a slow running gel means that the buffer is too concentrated so the increased salt means lower current and movement but you say that the voltage and current are good so I would check that the concentration of the acrylamide is correct ( not too high %) and that the cross linking is correct ( not too much Bis acrylamide) and try again ( just with size standard if the samples are valuable). Is anyone else in your lab having problems with this apparatus?
Have you check the Buffer running concentration? It happens usually when the Buffer is too much concentrate I guess.
Also, are you using the correct voltage (150V)?
Yes the voltage is as mentioned. I will replace the buffer in the next working day of the week and will check whether the problem is solved or not.........
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