P. aeruginosa Not Forming Biofilm on Lab-Tek—Possible Causes?

20 March 2025 0 579 Report

We tested a clinical strain of Pseudomonas aeruginosa for biofilm formation on Lab-Tek chambered slides in two separate assays: one using LB and another with TS + glucose. After 48h incubation, Live/Dead staining and CLSM showed only scattered aggregates, adherent, and planktonic cells, but no structured biofilm. However, crystal violet (CV) staining indicated biofilm formation, possibly on the well surface, which CLSM couldn’t visualize. - Could this strain require different conditions (e.g., medium, time, oxygen) for biofilm formation on Lab-Tek? - Could surface properties affect adhesion in this setup? - Any suggestions to improve biofilm formation in Lab-Tek chambers for CLSM? Any insights are appreciated. Thanks!

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