Goodmorning, I work with SEC-FPLC (column: Superose 6 Increase 10/300 GL) and I used it to run two different samples: 1) Cetuximab (145,78 kDa) and, as you can see from the image attached it elutes at 16.6 mL. The second sample is cetuximab functionalized with PEG (5000 Da each PEG molecule and the molar ratio between cetuximab and PEG is 1:20) which elutes at ml 14.8. Can you give an explanation of why CTX-PEG elutes so early comparing it with CTX alone and also with a sample of Heavt-chain ferritin (HFn, 509kDa, peak at 15.5 mL)? Does the PEG change the hydrodynamic volume of CTX so much?
Thank you
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