What is the reason of doing ovarectomy and then administer estrogen supplement to sustain the estrous cycle in endometriosis mice?
To prof the role of estrogen in endometriosis. Also to supplement accordingly need of study.
The ovariectomised rodent model is used to determine the role of ovarian hormones (estrogens and progesterone) in whatever thing is being studied. In most cases, it is preferable to ovariectomise all groups and have one group (or more as needed) with added estrogen at a fixed dose, as opposed to have intact animals (i.e. not ovariectomised) in the same experiment. This is because the ovary goes through cycles of more or less hormone release. For most experiments is more useful to know exactly the amount of estrogens the animal is exposed to.