Our sample has RIN value around 2 and we are able to amplify the target virus in PCR ! How important is RIN value for NGS of plant viruses?

01 January 2018 1 2K Report

Will be able to assemble virus genome, if we'll proceed with the same sample?

Thank you Mathew.

RIN was measured by the sequencing company. I have submitted my sample for NGS to the company and they provided me RIN Value.

The thing is, While i was working with the same sample for RT-PCR, with my own designed primers, we were able to amplify almost half of the genome from the same sample.

So my concern is, if the viral RNA is degraded , how come we were able to amplify! Will it not be possible to perform NGS on same sample?

Regards

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