I am facing consistency issue in Hac-1 Splicing?

I am studying Unfolded protein response in yeast and I am checking Hac-1 mRNA splicing through semi RT-PCR followed by PCR. But I did not found any similar pattern of Hac-1 splicing in at least...

03 September 2020 7,346 2 View

Preventive Maintenance Database failure?

I'm currently making an investigation of how to apply RCM with Preventative Maintenance to a truck fleet of fuel transport. In my company there's not a list of functional failure or failure modes,...

29 March 2020 5,769 3 View

Has anyone attended INTED (International Technology, Education and Development) conference in Valencia? What's your experience like?

I've been invited to present in INTED2020 and would be grateful for any piece of helpful information regarding attending the conference.

02 January 2020 3,448 2 View

Why does the brain neuron not utilize glucose directly ?

Brain needs glucose as main source of energy but needs non-neuronal cells to consume the glucose and convert it to some intermediates for brain use.

15 October 2019 5,242 1 View

How is glutamate recycling coupled to energy metabolism in the CNS ?

Glutamate is a neurotransmitter in the CNS.

15 October 2019 1,439 1 View

What would be the viable Research topics in Fiscal Policy and Monetory Policy?

I need research areas worth exploring in relation to Fiscal and Monetary Policies

15 September 2019 3,849 4 View

How can I modify the surface of silver Ag (CeO2) nano-particles with -COOH for EDC/NHS reaction?

I am looking for a working protocol to modify the surface of silver NP with carboxylic acid (because I want to use EDC/ NHS crosslinkers to link my NP to antibody). I have found an article related...

06 July 2019 5,914 2 View

Can anyone help me with link of e-book or pdf version of books related to "Tea Cultivation"?

I need book related to Tea Cultivation. If anyone have any links or pdf version of books related to tea cultivation, please help me with that. Thanks in advance. Prince

21 April 2019 9,931 2 View

How to fit a Birch Murnaghan 3rd order EOS in Matlab or Mathematica or any software ?

I have the data of energy vs distance from DFT calculation

12 March 2019 2,170 1 View

How to measure the accuracy of object tracking methods?

can i have the matlab code?

03 February 2019 1,922 8 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View