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Questions related from Bastian Fischer
Hello, I would like to establish an experimental setup for a generic two-organ-on-a-chip (OoC) system. Can the material (chamber, pump,...) needed for this be purchased commercially? Best...
21 December 2022 8,254 0 View
Dear all, I am doing some CRISPR experiments in neonatal fibroblasts. After a lot of optimization, the system works really well in these cells. As you will know, fibroblasts don´t really like to...
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Dear all, I've successfully used gRNA's that target the top DNA strand, but now I'd like to use a gRNA that targets the bottom strand. I just wanted to confirm that my cloning strategy is correct...
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Hey, I try to seperate different pyrophosphates via UPLC-MS/MS. For sure, their structure is very similar. For all of them, I measured the same product-ion...Is this a problem? I guess, it isn´t,...
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Dear all, I try to prove the presence of the LDL - receptor. I performed my Western Blot several times, with different antibodies and I always received the same bands. One of these bands (between...
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Dear all, I try to detect the LDL receptor in my liver Tumor cells (HEP - G2). For this. I have two different primary antibodies: 1) http://www.abcam.com/ldl-receptor-antibody-ab30532.html 2)...
07 May 2015 632 4 View
