We are trying to isolate bone marrow cells from bat long bones - the problem is that some samples have thick, very high fat content bone marrow, which makes it very hard to separate cells from the bone marrow mass. We tried forcing bone marrow through a mesh and mechanically 'chopping' it into smaller fragments but with little success. Just to give you better idea of how these several samples looked like: bone marrow mass was very hard, thick, difficult to flush from the bone, and when added to the growth medium it remained almost solid and buoyant, it did not release many cells. The attempted isolation took place within 24-48 hrs since death of animal and long bones were stored on ice until then. Perhaps the time of the year (bats preparing for hibernation) had something to do it the consistency of the samples? Anything you could recommend in such situation? Thank you very much for all suggestions and comments.
I'm not sure, but watch out for nasty viruses, especially Nipah and Marburg!
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