How could i use image j to count specific cells which are labeled by two FITC ab?

how should i adjust image j to count these cells automatically not manually? i put one of my slide image in attached file.

25 December 2020 9,598 2 View

What tests should I run for this small scale research?

25 October 2020 5,690 3 View

Why can't I see band in gel agarose while 260/280 ratio is 1.8 in Nanodrop?

Hi everyone I am trying to extract DNA from soil, 260/280 ratio is 1.8 in nanodrop, concentration is 587 ng/ul. but I can not see the band in gel agarose. Thank you in advance

18 October 2020 9,438 7 View

Does the alpha-synuclein overexpression in SH SY5Y cells have any toxic effect?

Cells are dying post-transfection. I've transfected SH SY5Y cells to overexpress alpha-synuclein, cells were overexpressed but they were dying rapidly. I've checked the overexpression, it was fine...

16 August 2020 7,518 3 View

Error while installing gromacs?

Hello all I was trying to install gromacs 2019 in an non-standard. but i countered this error: /usr/lib/gcc/x86_64-linux-gnu/5/include/mwaitxintrin.h(36): error: identifier...

16 June 2020 3,908 5 View

Is it mandatory to ask demographic details of the respondents for a qualitative research?

I am a novice researcher, working on my research paper these days. I want to know, can we do a qualitative research without asking demographic information of respondents. can we develop such a...

04 May 2020 5,799 16 View

Number of Antennas for Massive MIMO (Commercially+ acc. to 5G Standardization)?

Currently, most of the reports show that the no. of Tx/Rx antennas for mMIMO (commercially deployed and tested) are upto128 (64T64R-TDD etc). Does anyone knows: 1. What is the number of Tx/Rx...

19 March 2020 3,987 8 View

Could you help me with simulating drag force and dielectrophoresis force in comsol by using AC/DC module?

I'm trying to apply a nonuniform electric field and create a dielectrophoretic force in a channel and put a particle in the middle of the channel. I added these 2 physics, creeping flow and...

13 March 2020 9,721 3 View

What is a good research topic for MA thesis in education or teaching English?

Im thinking to do my thesis in an international level , kind of cross cultural topic. how can I find a grant from a foreign university ?

30 January 2020 5,548 3 View

How do I generate topology of many ligands which are not coonected all of them ,for gromacs?

Hi. I want to do simulate via gromacs for ligand- protein complex which there is 9 ligands (three of them are seperated and six of them are connected together(picture in Add files). when I used...

14 January 2020 5,707 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View