Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

HPLC_MS or GC_MS analytical method for acrolein, without sample pretreatements?

Can anyone suggest a GC-MS or HPLC-MS analytical method to identify Acrolein in a solid sample (soluble in ethanol), without any pretreatement of the sample (no purge and trap, no SPME)?

24 March 2020 3,223 9 View

BM-hMSC differentiation- Adiponectin ELISA

04 April 2017 4,075 3 View

Can anyone suggest me how can I test the activity of Wnt-3A conditioned medium with the top fop assay?

I'm using cell to produce Wnt-3a conditioned medium to growth organoid. How can I measure the activity of the medium ? I also would like to check the performance of my cells . Do they have limited...

03 October 2016 9,048 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

Can downdraft gasifier for RDF be scaled up?

Dear researchers, In literature, so far I am seeing that downdraft is usually used for small-scale purposes (10kW-10MW). One of the reasons behind is "they do not allow for uniform distribution...

08 February 2021 5,403 1 View

Which is the most ecofriendly and effective activated agent for the preparation of activated carbon from sewage sludge?

I am going to prepare activated carbon from the waste activated sludge coming out of WWTP. Can you please help me in understanding, which chemical/physical activating agent will be most...

07 February 2021 9,507 5 View