Stefan Zimmermann

Dear Dr. Zimmermann,

Thank you so much for your prompt and kind response!

Sincerely,

Sungho

Dear Sungho Jeong

We used PMA 50 ng/ml (Calbiochem, Billerica, MA) and 500 ng/ml ionomycin (Calbiochem) for C57Bl6/J derived T cells as per

Article A role for the histone H2A deubiquitinase MYSM1 in maintenan...

There might be some value to do a titration experiment as the genetic background of the mice you are using, the environment and the specific functional readout (certain cytokines, co-stimulatory molecules, proliferation) may all be somewhat affected by the concentration you are using.

All the best & good luck with your experiment,

Michael

Dear Dr. Michael Förster

Thank you for sharing your conditions and your suggestion! I will definitely do a titration experiment to optimize my experiment. Your reference and one above gives me a great idea of where I should be aiming for.

Thank you and good luck with your research too!

Sungho