Hi, Everyone I have a statistical issue.
I divided all my samples into type and age groups:
1. Young A;
2. Old A;
3. Young B;
4. Old B.
I need to compare A vs B under young and aged groups, separately. At the same time, I need to compare young vs old under A and B groups, respectively.
Based on my statistical knowledge, it seems that I have more than two groups so ANOVA should be my test. However, all my groups could be regarded as two independent and unconnected groups and in each group, I compare two sub-groups (because I never compare Old A vs Young B or Young A vs Old B). So Actually I should use independent t-test or Mann Whitney U test.
However, when I look into similar literature, they prefer ANOVA, which really confuses me.