The middle sample is the first morning urine for proper testing. Usually, at least 3 morning urine samples on three different days in separate sterile containers recommended by the laboratory are required to verify the presence of TB germs. Because antibiotics inhibit the growth of TB germs in the urine, it is advisable that the patient does not take antibiotics before giving the sample. Samples less than 40 ml or held for more than 24 hours will not be suitable for culture. It is difficult to see TB germs in the urine sample and up to seven samples are recommended to increase the probability.