I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles?
@Macolm Nobre thank you for your answer, actually my question is how many total cells needed, beacause i tried with 10 dishes (of 1.5-2 million) as you said, but at final i had nothing after ultracentrifugation, so how many dishes do I need?