Nait Eldjoudi Amina

5 Questions 12 Answers 0 Followers

Questions related from Nait Eldjoudi Amina

How to find a gene primer sequence that detect only the human and not mouse gene?

Hello, I would like to do qRT-PCR to look for a specific gene in a human metastasis in mouse brain samples, so I need a sequence that only recognise human gene and doesn't cross with mouse genome....

20 April 2022 4,220 2 View

Best method for labelling EVs?

What is the best method of labelling extracellular vesicles for in vitro experiences?

27 June 2021 4,387 2 View

Does anyone use ImageJ Fiji to analyse HUVEC angiogenesis?

I have some questions about the analysis: - can we analyse image taken with OBJ4X or we should only use 10X images? -how many images per well (96plate) do you take? shall i take images to have the...

21 March 2021 3,507 3 View

Number of cells for extacellular vesicles extraction?

hello! I want to extract vesicles from MDA MB 231 cell ligne, culture in serum free media for 48h, would you help me with th number of cells required to have the vesicles? thank you

04 December 2020 6,630 2 View

Hi, I think to use albumin as purity control of Extracellular vesicles isolated from culture cells,shall i use Bovine or human Antibodies?

MISEV 2018 recommands Albumin, but since i use FBS for my culture, shall i look for Bovine Serum Albumin co_isolation? thank you

23 October 2020 4,304 5 View