Nucleus penetrating peptide -collaboration?

01 December 2017 1 8K Report

Recently we have synthesized several CPP (cell penetrating peptide) and one of them appeared to be located straight into nucleus of the cell (fibroblast and keracynotes cell line). As we are chemist/biochemist sitting on the edge of cell biology we are looking for cooperation to investigate properties of this peptide and possible mechanism and so on. Please send me a private message if interesting.

best

Adam

Tahereh Ebrahimi

hi dear Mr. adam lesner.

i want to work in cpp-mediated delivery of pDNA encoding toxic gene ... For PhD thesis .

and i intend to design a cpp that can stably transfer the plasmid to nuleus.

can you help me?

Badges
Science topic

More Adam Lesner's questions See All

Phengaris rebeli on Picea abies?

Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...

15 July 2024 8,099 4 View

Please tell me the scientific guidelines (books) on Trombidiformes ticks?

books and scientific papers on the morphology, taxonomy and so on of ticks of the suborder Trombidiformes

17 June 2024 948 2 View

Do plants produce hormones that have the same influence properties of animal hormones?

I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?

13 June 2024 3,860 1 View

Micelle vs liposome?

I hope we will not need to reinvent the wheel. Quick (maybe not very smart) question: How do you distinguish between these tw particles? Any proposed technique will definitely provide the answer....

21 May 2024 9,073 5 View

Why there is a increase in participants in road running events?

There is a huge demand for road running events such as marathons in past couple of years. why?

01 May 2024 3,612 0 View

Which of the following proliferation-tracing dyes perform the best?

I'm designing a cytotoxicity assay to measure the effect of treatment on proliferation. My specific objective is to compare the number of cells with 1 and 2 divisions in treated cultures to...

24 April 2024 2,566 0 View

Cointegration in case of the mixture of I(0) and I(1) stationary variables?

Hi! I want to use the ADL model for my data analysis. However, after performing a stationary test, dependent and 6/8 independent variables are stationary only in differences. The other two are...

23 April 2024 9,017 3 View

"Abaqus/standard analysis exited with an error". Is there a solution?

Hello, I am currently trying to run a simulation for my research using abaqus. I have installed abaqus 2022 version and also followed the required steps to link abaqus with intel fortran. I have...

15 April 2024 3,123 1 View

What is the procedure for implementing the DDMRP methodology on SAP S/4HANA and how to do it?

i'm an intern and this is what i'm suposed to do

15 April 2024 6,048 0 View

Dear scholars, could you please share with me free resources (e.g., Online courses and text books) about mulitilevel Linear Modeling?

Any kind of resources.

11 April 2024 9,293 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View