What is the technique being used? We use ProteinTech for Western blots to detect Nrf2.
1. Ensure that the antibody dilution buffer is correct and compatible with the assay being performed. 2. Make sure that the antibody has been stored and handled properly and that the expiration date has not passed. 3. Check the antibody concentration and make sure it is not too dilute or too concentrated. 4. Perform a titration of the antibody to determine the optimal concentration for the assay. 5. Perform a western blot with a known positive control to ensure that the antibody is working correctly. 6. Cross-check the antibody's literature to ensure that it has been tested for the experiment in question. 7. Perform a pre-adsorption test to make sure that the antibody does not recognize any other proteins in the sample. 8. If the antibody is not working, try using a different clone or isotype. 9. Consider performing another assay, such as an ELISA or IHC, to double-check the results. 10. Consult technical support or customer service for further assistance.
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