TLDR - I've been working on creating frozen sections of bone. Norland Optical Adhesive 63 (UV curing agent) always turns white during immunofluorescence protocol, and my sections become practical unusable. Any suggestions for preventing this?

The process is as follows:

fixation in 4% PFA for 48 hours, cryoprotection in 30% sucrose overnight, then embed in OCT and section block. We do not decalcify our bones for frozen sections. As such, we have to use the tape transfer system in order for sections to stay on the slide. For sectioning, I have been putting a very thin layer of Norland Optical Adhesive 63 on the slide, transferring the section of bone (15um) to the slide via tape and rolling it out, flashing the slide 2x with UV and letting dry at RT for ~5 minutes before taking the tape off. I store in -20°C. I want to stain these sections using immunofluorescence, but every time I incubate the sections in primary or leave them in a humid/moist environment, everywhere the glue was turns white. I cannot see my sections well and my staining looks terrible because of it.

Has anybody had any experience with this problem and knows how to prevent the glue from turning white? I've attached a picture as an example.

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