How can I validate the UV spectroscopy?

can I compare UV spectroscopy reading with nanodrop reading to validate the UV spectroscopy? or is there a better way to know if the UV works well or not? Note: we use it in DNA, RNA &...

12 December 2020 4,869 2 View

What's the right way to Tween 20 storage?

02 December 2020 9,955 2 View

What's makes RNA (and not DNA) bind to the silica spin column in commercial kits?

In silica spin column-based kits, if the spin column material is the same and the binding to the column occurs by using ethanol and Guanidine salts. Then, what's the difference between the RNA...

20 September 2020 4,798 1 View

Why Non specific bands of PCR reaction appear while using specific primers for tobamoviruses?

i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...

09 December 2019 7,276 3 View

How can measure the concentration of dopamine and serotonin in rat brain tissue?

How can measure the concentration of dopamine and serotonin in rat brain tissue?

11 December 2017 6,461 10 View

Help Please!! how to solve the error "too many attempts made for this increment" in abaqus?

i am applying a load of 50KN gradually on a reinforced concrete beam, i'm unloading this load using an amplitude to get the loading and unloading versus deflection graph.i have used concrete...

09 June 2017 3,129 14 View

Hello How to get a producer to dissolve cellulose fiber by carbon disulfide ?

11 April 2017 9,899 2 View

How to get the frequencies of simply supported RC beam when changing load in ABAQUS?

i have modeled the RC beam with simply supported BC and used concrete damaged plasticity model to account for the nonlinearities in concrete, i have got the frequency value for the case of beam...

01 January 2017 737 6 View

What kind of experiment help in understanding the metabolic pathway in plants?

In my proteomic results, there is change in glycolysis and TCA and i want to know that which pathway is involved in changing these major metabolic processes. For that i want to do some more...

18 September 2016 396 3 View

What are the problems in teaching and learning speaking skills at undergraduate level?

I want to know the current practices and coping strategies of teaching speaking skills as well.

17 June 2016 3,467 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View