How to Loop a macro in imagej?

Dear All, hi I would like to loop a micro, my micro below: run("Grid/Collection stitching", "type=[Filename defined position] order=[Defined by filename ] grid_size_x=2 grid_size_y=2...

01 February 2020 1,852 1 View

How to fill holes with dipimage when analyzing images with Matlab?

Dear all i am a starter in image analyzing using matlab. I am using dipimage tool in matlab to analyse images from cells. I need to have cytoplasm compelete. But there are Some vesicles that are...

07 August 2019 9,284 5 View

How to convert DNA seq to drawing ?

Hi I am wondering if there is a website/software to convert DNA seq to drawing like the photo attached? kind regards somaieh

03 April 2019 153 2 View

JC-1 bleaching in confocal imaging?

Dear All hi I am doing live cell confocal imaging with different cell line using JC-1 probe for mitochondria, I noticed very fast bleaching. i reduced bleaching using resonance scanning. As...

03 April 2019 3,375 3 View

How to define resolution of image in igore?

02 March 2017 939 0 View

Does anyone have experice working with Igor pro?

I need to draw a graph with three sets of data for two groups, but it is not working for me. I know how to do it when i need only one set of data. i will be thankful receiving some advice :-)

01 February 2016 7,071 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View