No library produced after KAPA mRNA stranded library prep. Any help?

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How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

Problem Justifcation of below research topic?

Analyzing the effectiveness of apply the Extended Producer Responsibility (EPR) concept for reducing pre-consumer apparel waste within a Star Fashion Pvt Ltd apparel company.

30 June 2024 3,764 0 View

Anyone familiar with the Bruker in-vivo F-PRO Photodocumentator?

Hello! I'm a PhD student and we need urgent technical help for the "Multimodal Pro Light Source 400W" equipment. We are having image acquisition problems in our laboratory. When we activate...

16 May 2024 9,545 0 View

Critically discuss complexity theory and the notion of resilience as they relate to public policy?

Explain the concept of complexity theory Discuss the link between resilience and complexity theory Explain how these relate to public policy illustrate your discussion with appropriate example

05 April 2024 8,886 1 View

Testing methods to determine a non-gene-silencing enzyme inhibitor?

I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...

16 March 2024 392 1 View

What evaluate to choose the best docking program for my protein and ligands?

I performed virtual screening of some molecules in Auto Dock Vina and GOLD and obtained different results for the best ligand. I'm not sure if I did something wrong, or the docking program is not...

07 March 2024 3,680 3 View

How to separate a misture of coordenation coumpounds that are water-soluble?

I did a synthesis of a carbonyl ruthenium complex coordinated with the 1H-imidazole-1-ylacetic acid, but I'm having problems in the purification process. All the mixture is only slightly soluble...

03 March 2024 2,041 3 View

Continuing PCR with contaminated reagents?

I want to test out some new primers, but I know from past PCRs that at least one of my reagents is contaminated. I'm on a time crunch to finish my project and am not sure if I can get new reagents...

21 February 2024 4,799 0 View

Can the same primers be used for different cell lines from the same species?

If primers amplify a specific region of DNA, and the same DNA is present throughout the body, can a single primer be used across different cell lines and different organs from the same species?

23 January 2024 6,465 2 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

How to add Cr parameter in autodock?

When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!

29 July 2024 488 0 View

How to fix install package meta in R?

> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’

18 July 2024 3,536 0 View

Language teachers can develop speaking skill through listening practices. Can you please suggest some more ways to promote the same (Speaking Skill)?

"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...

01 July 2024 8,406 5 View

Are there different databases similar to the Zinc database or how can I solve this problem in the Zinc database?

We were using the Zinc database for the virtual compound library in our studies, but there have been problems downloading a large number of compounds for a while. Are there different databases...

30 June 2024 8,144 1 View

Can I directly ligate Illumina adaptor to the end of ssDNA?

I have a sequence of ssDNA that I would like to directly ligate an Illumina sequencing adaptor to. As long as I have done the end repair (remove phosphates), can I directly ligate the adaptor to...

26 June 2024 4,522 0 View

Are there standard libraries/frameworks for doing RLHF for training LLMs ?

When it comes to Re-inforcement Learning with Human Feedback, are there standard libraries/frameworks for training LLMs ?

25 June 2024 1,121 0 View

Do we have any python package for implementing contexual bandits (used in Recommendation Systems) ?

How can contexual bandits (used in Recommendation Systems) be implemented in code via library package ?

18 June 2024 3,196 6 View

Best Tools for Wrist PPG Signal Analysis: Your Recommendations?

I'm conducting research on photoplethysmography (PPG) signals obtained from smartwatches worn on the wrist. The main goal is to analyze the PPG waveform and extract key fiducial points and...

13 June 2024 3,286 0 View

I am writing to submit my article for possible publication in ,” African Journal of Library, Archives and Information Science” ?

I don't know how to submit my article?, then I inform you that I registered with Ajol . Thank you in advance for your attention to this submission

10 June 2024 2,652 3 View